THE EFFECTS OF DIFFERENT STORING TEMPERATURES ON THE ACTIVITY OF SHEEP BLOOD AND PLASMA GLUTATHIONE PEROXIDASE

Abstract

The aim of the study was to determine the stability of the activity of
glutathione peroxidases 1 and 3 during storage at +40C and -180C. Blood samples
were taken from eight sheep and the activity of the enzyme was determined in the
plasma (GPx3) and erythrocytes (GPx1) on the first, third, fifth and seventh day in
samples stored at +40C and after one and three months in samples stored at -180C.
GPx3 activity decreased significantly during storage at both temperatures, while
GPx1 remained steady even after three months of storage at -180C. Obtained results
indicate that GPx3 activity has to be determined in fresh sheep plasma samples,
while the activity of sheep erythrocyte GPx1 can be determined even after 3 months
of storage at -180C.