EFFECT OF SOMATIC ANISAKIS SIMPLEX EXTRACT TO DEVELOPMENT CHICK EMBRYOS

Abstract

Helminthes have embryotoxic and teratogenic effects to host’s cells and tissues.
Investigations were carried out mainly on embryo and fetus in mammals.
Blaszkowska, J. (1998); Kadlubowski R. (2000) conducted studies of in chick
embryos. Dose-dependent effect is also studied and found that small doses cause
embryotoxic effect, while higher doses - teratogenic. Anisakis simplex antigens
have also embryotoxic effect has been found. The aim of our research was to
examine the effect of A. simplex somatic antigen to the development of chick
embryos in its experimental introduction in various ways and at different stages of
embryogenesis. For the study chicken embryos were used at different stages of
embryogenesis: 7.5, 10.5 and 12.5 days. Methods for introducing antigen chicken
embryos: in the allantois cavity, on-chorion allantois membrane and yolk sac at 0.2
ml. The autopsy was performed on embryos 2 and 8 days. The eggs and embryos
were weighed and evaluated the embryonic development. As a result of the first
experience the greatest change observed by mass, and when administered in the
development of antigen in both yolk sac on day 2 (1.68 ± 0.06, unlike control 2.25
± 0.20), and at 8 days ( 9.97 ± 6.21 in the experiment versus 14.89 ± 0.78 in the
control), respectively. In the second experiment eggs were used at 1 and 5 day
incubation embryos. The antigen was administered in both cases also in the yolk
sac at a dose of 0.2 ml. In the second experiment there was a delay in the
development of both cases at autopsy after 2 days in non-incubated eggs missing
the development of, unlike the control, the development of which 48 hours
corresponded; including 5 daily observed decrease in weight 0.88 ± 0.22, against
1.05 ± 0.05 and 8 days in a similar way. As a result of experiments on the effect of
the Anisakis simplex somatic antigen on the development of chick embryos
embryotoxic action installed. Thus antigen has the greatest effect on development
of early embryos and putting it into the yolk sac.