EFFECTS OF POLLEN CONTAMINATION AND KERNEL WEIGHT ON KERNEL STRUCTURE OF MAIZE IN OPEN AND SELF POLLINATION TREATMENTS
In small plot experiments conducted in maize, the mostly used pollination methods
are open and self-pollination treatments. Comparative studies using these
treatments are abundant in scientific literature; however studies on the effect of
cross pollination and kernel weight on kernel biochemical properties in different
treatments are limited. In this study, we conducted a comparative experiment to
investigate the effect of pollen contamination and mean kernel weight on kernel
biochemical composition of ten different maize genotypes by using two different
pollination methods.Open and self-pollination treatments were applied as
pollination methods. Eight different traits; kernel weight, cross pollination rate,
protein, oil, carbohydrate, oleic acid linoleic acid and carotenoid content were
measured. Regression analyses were performed to understand the effects of cross
pollination and mean kernel weight on biochemical constituents of maize kernel in
different treatments. Results showed that the major biochemical traits, such as
protein, oil and carbohydrate content were significantly affected by pollen
contamination but minor traits were not. When data were combined (n=60) the
effect of pollen contamination in different treatments was not clearly understood.
When data (n=30) of each treatment were separately analyzed, it was found that
cross pollination rate had significant effect on the most of biochemical constituents
in open pollination. Overall, results suggested that pollen contamination had an
effect on major biochemical traits in maize and hand pollination could be used for
preventing of unwanted effect of pollen contamination in small plot experiments.
However, it should be considered that the effects of hand pollination on kernel
weight affect the some biochemical traits in maize.