BOTTLENECK ANALYSIS OF ANATOLIAN BLACK CATTLE (BOS TAURUS) USING MICROSATELLITE MARKERS

Abstract

The present study was conducted in order to reveal the genetic diversity and
bottleneck in Anatolian Black Cattle (Bos Taurus). Animal material of the study
consisted of 75 cattle raised in International Center for Livestock Research and
Training. The bottleneck in the cattle breed studied was checked with 10
microsatellites markers, amplified in a multiplex polymerase chain reaction (PCR)
were used according to recommendation of FAO (2011). A total of 116 alleles was
observed from microsatellites studied. Overall value belongs to average number of
alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho),
expected heterozygosity (He), the polymorphic information content (PIC), average
heterozygosity (Ĥ), and FIS, known as the inbreeding coefficient, were 11.60, 5.35,
0.80, 0.78, 0.80 and 0.012, respectively. All microsatellite markers except INRA23
and ETH3 deviated from Hardy Weinberg equilibrium (HWE). Bottleneck was
analyzed with Bottleneck software according to three different mutation models
including the infinite allele model (IAM), two-phase mutation model (TPM) and
stepwise mutation model (SMM). It can be said that there is not any ultimate risk in
terms of bottleneck considering L–shaped curve showing normal distribution
obtained from the analysis.