COMPARISON OF HPLC AND UHPLC METHODS IN PHARMACEUTICAL ANALYSIS

Abstract

Modern chromatographic methods are widely used in the development and quality control of active pharmaceutical substances and dosage forms. High-performance liquid chromatography, which is suitable for qualitative and quantitative analysis, is mostly used. However, in recent years, the relatively new technique of liquid chromatography under ultra-high pressure, which has retained the advantages of classic high-performance liquid chromatography, has been increasingly used, but has been additionally improved with more efficient chromatographic performance. The paper compares these two modern, efficient and in principle very similar chromatographic methods, which found extensive use in the analysis of active pharmaceutical substances and dosage forms. It is important to perceive the differences in the application of these two methods in routine analysis, guided by scientifically based evidence for their advantages and disadvantages. A great contribution was also made by the stationary phases, which have particles of small dimensions that can withstand greater pressure compared to classical stationary phases, and therefore it is necessary to make additional adjustments to the system.