GENOTOXIC EFFECT OF NEWLY SYNTHESIZED NANOMATERIALS FOR POTENTIAL DENTAL APPLICATION

Abstract

Introduction: Biocompatibility is the property of a material to fulfill its function in the body without causing harmful side effects. To confirm biocompatibility, all newly synthesized materials must undergo numerous in vitro and clinical tests. The starting point is cytotoxicity and genotoxicity tests. This study aimed to determine the genotoxicity of newly synthesized nanomaterials based on calcium aluminates and calcium silicates with additional hydroxyapatite. Material and methods: The research was conducted at the Institute of Oncology in Belgrade. An alkaline version of the Comet test was used, following the recommendation of the international standard ISO / DIS 10993-3, and MRC-5 (normal human lung fibroblasts) was used as a cell line. Cells were grown in monolayer culture, in a complete nutrient medium, at a temperature of 37 °C in air enriched with 5% CO2 and saturated water vapor. The genotoxicity of calcium aluminate and mixtures of hydroxyapatite and calcium silicate was investigated. and untreated cells were used as controls. A 40x lens on a fluorescence microscope was used to visualize the DNA damage. Comet Assay IV (Perceptive Instruments) was used to statistically process the results. Results: ALBO-CA extract did not show a genotoxic effect, that is, the percentage of DNA damage at the highest applied concentration of cement of 100 mg / mL, was 9.6%. ALBO-CS-HA extract showed genotoxic potential at concentrations ≥ of 25 mg / mL Conclusion: Nanostructured calcium aluminate did not show genotoxic potential on human lung fibroblasts, unlike ALBO-CSHA, with recommendations for further studies.