• Branka Rodić Grabovac University of Banja Luka, Faculty of Technology, Bulevar Vojvode Stepe Stepanovića 73, Banja Luka, Republic of Srpska
  • Radana Đuđić University of Banja Luka, Faculty of Technology, Bulevar Vojvode Stepe Stepanovića 73, Banja Luka, Republic of Srpska
  • Pero Sailović University of Banja Luka, Faculty of Technology, Bulevar Vojvode Stepe Stepanovića 73, Banja Luka, Republic of Srpska




Obtaining of biologically active cellulosic bandage with antimicrobial activity is one of the most interesting trends in the development of new medical materials. This paper investigates the effect of reaction conditions on oxidation of cellulose fiber in a form of bandage with an aim to obtain a material suitable for chemical binding of antibiotics. Also, the influence of structure of cefazoline on sorption on oxidized cellulose fiber is investigated. The chemical bonding of the drug was performed on oxidized cellulose samples ( OC ) with different carboxylic groups content in cefazoline water solution concentration with c = 1.7 ∙ 10-3 mol / L, while the desorption of bonded antibiotic was done in physiological solution. The amounts of bonded and released drug were determined spectrophotometrically in UV range. It was found that carboxylic groups content on OC and solution pH during the sorption process had a significant influence on the amount of bonded antibiotics. Antimicrobial activity of fiber samples with bonded cefazoline was tested in vitro in relation to gram positive and gram negative microorganisms (Staphylococcus aureus, Bacillus subtilis and Escherichia coli). The paper showed that the optimal ratio of cefazoline consumption versus the amount of released antibiotic was obtained for the sample with 6.48 % of the carboxylic group.


[1] B. Rodić-Grabovac, R. Đuđić, Lj. Topalić-Trivunović, M. Balaban, Antimicrobial activity of modified cellulose fiber with bonded cephalexin monohydrate [In Serbian: Antimikrobno djelovanje modifikovanog celuloznog vlakna sa vezanim cefaleksin monohidratom], Glasnik hemičara, tehnologa i ekologa Republike Srpske, Vol. 6 (2012) 1−9.

[2] B. Rodić-Grabovac, R. Đuđić, N. Ilišković, The bonding of anesthetics and antibiotics on carboxymethylcellulose [In Serbian: Vezivanje anestetika i antibiotika na karboksimetilcelulozu], Hemijska industrija, Vol. 61−4 (2007) 203−207.

[3] J. Buchenska, S. Slomkowski, J. Tazbir, E. Sobolewska, Antibacterial Poly(ethilene teraphthalate) Yarn Containing Cephalosporin Type Antibiotic, FIBERS & TEXTILES in Eastern Europe, Vol. 11−1 (2003) 40.

[4] J. Buchenska, S. Slomkowski, J. Tazbir, E. Sobolewska, Poly(ethylene terephtalate) yarn with antibacterial properties, Journal of Biomaterials Science, Polymer edition, Vol. 12 (2001) 55−62.

[5] M. Vuorio, J. A. Manzanares, L. Murtomaki, J. Hirvonen, T. Kankkunen, K. Kontturi, Ion-exchange fibers and drugs: a transient study, Journal of Controlled release, Vol. 91 (2003) 439−448.

[6] A. Medović, P. Škundrić, M. Kostić, I. Pajkić-Lijaković, The mathematical model of insulin desorption from the bioactive, fibrous artificial store, Journal of Biomedical Materials Research, Vol. 79 (2006) 635−642.

[7] T. Saito, A. Isogai, Ion-exchange behavior of carboxylate groups in fibrous cellulose by the TEMPO-mediated system, Carbohydrate Polymers Vol. 61 (2005) 183−190.

[8] L. Zhu, V. Kumar,. G. S. Banker, Examination of Aqueous Oxidized Cellulose Dispersions as a Potential Drug Carrier. I. Preparation and Characterization of Oxidized Cellulose-Phenylpropanolamine Complex, AAPS PharmSciTech Vol. 5−4 (2004); Article 69.

[9] A. F. Zappala, W. W. Holl, Post Analytical Profiles of Drug Substances, Volume 4, Edited by Klaus Florey, The Squibb Institute for Medical Research New Brunswick, New Jersey 1975, 1−20.

[10] V. Kumar, T. Yang, HNO3/H3PO4-NaNO2 mediated oxidation of cellulose-preparation and characterization of bioabsorbable oxidized celluloses in high yields and with different levels of oxidation, Carbohydrate Polymer, Vol. 48 (2002) 403−41.

[11] USP (United States Pharmacopeia 23/National Formulary 18) (1995) Oxidized cellulose, p. 318

[12] R. L. Whistler, Methods in Carbohydrate Chemistry, Vol. III, Cellulose, Academic Press, New York and London, 1963, 49−51.

[13] J. H. Ortez, Disc diffusion testing, In Coyle MB, Coordinating editor. Manual of Antimicrobial Susceptibility Testing, American Society of Microbiology (2005) 39−52.