OBTAINING OF MATERIALS WITH ANTIBACTERIAL ACTIVITY BY BONDING OF CEFAZOLINE ON MODIFIED CELLULOSIC BANDAGE
Obtaining of biologically active cellulosic bandage with antimicrobial activity is one of the most interesting trends in the development of new medical materials. This paper investigates the effect of reaction conditions on oxidation of cellulose fiber in a form of bandage with an aim to obtain a material suitable for chemical binding of antibiotics. Also, the influence of structure of cefazoline on sorption on oxidized cellulose fiber is investigated. The chemical bonding of the drug was performed on oxidized cellulose samples ( OC ) with different carboxylic groups content in cefazoline water solution concentration with c = 1.7 ∙ 10-3 mol / L, while the desorption of bonded antibiotic was done in physiological solution. The amounts of bonded and released drug were determined spectrophotometrically in UV range. It was found that carboxylic groups content on OC and solution pH during the sorption process had a significant influence on the amount of bonded antibiotics. Antimicrobial activity of fiber samples with bonded cefazoline was tested in vitro in relation to gram positive and gram negative microorganisms (Staphylococcus aureus, Bacillus subtilis and Escherichia coli). The paper showed that the optimal ratio of cefazoline consumption versus the amount of released antibiotic was obtained for the sample with 6.48 % of the carboxylic group.
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