• S. Janjić University of Banja Luka, Faculty of Technology, Vojvode Stepe Stepanovića 73, 78 000 Banja Luka, Republic of Srpska
  • M. Kostić University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia
  • P. Škundrić University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia
  • B. Lazić University of Banja Luka, Faculty of Technology, Vojvode Stepe Stepanovića 73, 78 000 Banja Luka, Republic of Srpska
  • J. Praskalo University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia



Cellulose and chitin are the most abundant polysaccharides in nature. Chitin is the natural amino polysaccharide and is estimated to be produced annually almost as much as cellulose. These resources are renewable and inexhaustible if rationally utilised. Unique properties of chitin and chitosan (chitin derivative obtained by the deacetylation of chitin), such as antibacterial activity, biocompatibility, non-toxicity and bioresorptivity make these materials very suitable and important biomaterials. During our studies, we examined the possibilities of obtaining composite, biologically active cellulose-chitosan fibres. Аn effective two-stage procedure for obtaining antibacterial fibers based on cellulose and chitosan was developed. The first stage involves the formation of dialdehyde cellulose by potassium periodate oxidation of cellulose fibers, which is able to form Schiff’s base with chitosan. In the second stage, chitosan coated cellulose fibers were prepared by subsequent treatment of oxidized cellulose fibers with a solution of chitosan in aqueous acetic acid. Maximum percentage of chitosan introduced into/onto the cellulose fibers was 0,51 % (w/w). Antibacterial activity of cellulose fibers coated with chitosan as the active component against bacteria Escherichia coli and Staphylococcus aureus, was confirmed by in vitro experiments.


[1] D. Klemm, B. Heublein, H-P. Fink, A. Bohn, Cellulose: Fascinating biopolymer and sustainable raw material, Angew. Chem. Int.Ed,. Vol. 44 (2005) 3358−3393.

[2] M. N. V. Ravi Kumar, A review of chitin and chitosan applications, Reactive&Functional Polymers, Vol. 46 (2000) 1−27.

[3] K. Kurita, Controlled functionalization of polysaccharide chitin, Prog. Polym. Sci., Vol. 26 (2001) 1921−1971.

[4] S. W. Shalaby, Biomedical Polymers, Designed-to-Degrade Systems, Hanser/Gardner Publications, Inc, Cincinnati 1994.

[5] R. D. Gilbert, Cellulosic Polymers, Blends and Composites, Hanser/Gardner Publications, Inc., Cincinnati 1994.

[6] R. A. A Muzzarelli et al., Chitin nanofibrils/chitosan glycolate composites as wound medicaments, Carbohydrate Polymers, Vol. 70 (2007) 274−284.

[7] Y. C. Chung, Y.-P. Su, C. C. Chen, G. Jia, H.-L. Wang, J. C. G. Wu, J.-G. Lin, Relationship between antibacterial activity of chitosan and surface characteristics of cell wall, Acta Pharmacologica Sinica, Vol. 25(7) (2004) 932−936.

[8] H. K. No, N. Y. Park, S. H. Lee, S. P. Meyers, Antibacterial activity of chitosan and chitosan oligo-mers with different molecular weights, International Journal of Food Microbiology, Vol. 74 (2002) 65−72.

[9] G. M. Mikhailov, M. F. Lebedeva, L. A. Nud`ga, V. A. Petrova, Composite fibers based on chitin and cellulose, Russ. J. Appl. Chem., Vol. 74(9) (2001) 1573−1576.

[10] S. Strnad, O. Sauper, A. Jazbec, K. Stana-Kleinschek, Influence of chemical modification on sorp-tion and mechanical properties of cotton fibers treated with chitosan, Text. Res. J., Vol. 78 (2008) 390−398.

[11] X. D. Liu, N. Nishi, S. Tokura, N. Sakairi, Chitosan coated cotton fiber: preparation and physical properties, Carbohydr. Polym., Vol. 44 (2001) 233−238.

[12] T. P. Nevell, The Mechanism of the Oxidation of Cellulose by Periodate, Journal of the Textile Institute, Vol. 48(12) (1957) T484−T494.

[13] A. Potthast, M. Kostic, S. Schiehser, P. Kosma, T. Rosenau, Studies on oxidative modifications of cellulose in the periodate system: Molecular weight distribution and carbonyl group profiles, Holzforschung, Vol. 61 (2007) 662−667.

[14] H. S. Seong, J. P. Kim, S. W. Ko, Preparing chito-oligosaccharides as antimicrobial agents for cotton, Textile Res. J., Vol. 69(7) (1999) 483−488.

[15] Z. Zhang, L. Chen, J. Ji, Y. Huang, D. Chen, Antibacterial properties of cotton fabrics treated with chitosan, Textile Res. J., Vol. 73(12) (2003) 1103−1106.

[16] SRPS H.N8.132:1966, Celuloza - Određivanje bakrovog broja.

[17] J. Röhrling, A. Potthast, T. Rosenau, T. Lange, A. Borgards, H. Sixta, at al, A novel method for the determination of carbonyl groups in cellulosics by fluorescence labeling. 2. Validation and applications, Biomacromolecules, Vol. 3(96) (2002) 9–75.

[18] SRPS-ISO 937:1992, Određivanje sadržaja azota (Referentna metoda).

[19] D. Höfer, Antimicrobial textiles–evaluation of their effectiveness and safety, Hipler&P.Elsner (Eds.), Biofunctional textiles and the skin, Basel, Switzerland: Karger AG., 2006., 42–50.

[20] S. Z. Rogovina, L. K. Golova, O. E. Borodina, G. A. Vikhoreva, Chitosan—Cellulose Films Fabricated from Mixtures of Polysaccharides in N-methylmorpholine N-oxide,Fibre Chemistry, Vol. 34(1) (2002) 18−20.

[21] P. Calvini, G. Conio, E. Princi, S. Vicini, E. Pedemonte, Viscometric determination of dialdehyde content in periodate oxycellulose. Part II. Topochemistry of oxidation, Cellulose, Vol. 13 (2006) 571−579.

[22] A. J. Varma, V. B. Chavan, A study of crystallinity changes in oxidised celluloses, Polym. Degrad. Stabil., Vol. 49 (1995) 245−250.

[23] U. J. Kim, S. Kuga, Reactive interaction of aromatic amines with dialdehyde cellulose gel, Cellulose, Vol. 7 (2000) 287−297.

[24] Q. P. Zhong, W. S. Xia, Physicochemical Properties of Edible and Preservative Films from Chito-san/Cassava Starch/Gelatin Blend Plasticized with Glycerol, Food Technol. Biotechnol., Vol. 46(3) (2008) 262−269.

[25] Q. Hou, W. Liu, Z. Liu, B. Duan, L. Bai, Characteristics of antimicrobial fibers prepared with wood periodate oxycellulose, Carbohydrate Polymers, Vol. 74 (2008) 235−240.