• Ljubiša Petrov
  • Lidija Matija



One of the most perspective available techniques for investigation of the composition, structure and properties of materials, is scanning probe microscopy (SPM), respectively its components scanning tunneling microscopy (STM) and atomic force microscopy (AFM). This technique is used in multidisciplinary research in the field of medicine, pharmacy, dentistry, material science, etc., for study of biological samples, chemical compounds, pharmaceutical products, artificial tissues, implantology materials, and all other materials that have nanotechnological impact on application in these scientific fields. This is because the probes have not perfect size and geometry, which leads to the appearance of artifacts. They are defined as characteristics that appear on the image and are not present on the sample. These effects caused by convolutions between the probe and sample can be corrected to a certain extent by mathematical manipulation of topographic data. The methodology used in this paper is based on algebra of sets, and basic tools of mathematical morphology. Mathematical algorithms for the „blind reconstruction“ of the tip were used, and then in order to detect the parts of the sample surface which is not available in real-time scanning deconvolution was applied. The limit of the real probe tip is calculated from the image, using the morphological limitations inherent in the recording process. The result acuired as an image of the reconstructed surface out of the used images, with the reconstruction of the real tip. The presented results are clear proof of the usability of atomic force microscopy as a technique for imaging of biological materials on nano-level, and the applied algorithms increase the usability of the images in terms of a better conclusion based on precise numerical data taken from the processed images.
Keywords: Characterization, materials, SPM, AFM, probes, artefacts, convolution, post-processing, emulation.



link situs slot gacor terbaru Situs toto togel 4D Toto Situs agen toto togel 4D Situs toto togel terpercaya slot deposit pulsa bandar togel 4d bandar togel 4d situs togel slot bandar togel resmi bandar togel 4d slot online gacor bandar slot pulsa situs togel 4d situs togel macau bandar togel macau situs togel hk judi slot online situs toto togel 4d situs toto togel keluaran toto togel slot togel 4d situs toto togel toto togel resmi togel resmi 4d bandar togel 4d toto macau 4d bandar togel 4d slot deposit pulsa situs toto togel 4d situs togel terpercaya situs toto togel situs slot online situs togel 4d situs togel toto toto togel 4d slot togel 4d situs togel terpercaya togel toto slot bandar togel terpercaya toto togel situs toto 4d resmi situs togel toto 4d bandar toto macau terpercaya bo toto togel 4d resmi