Comparison of DNA Yield and Quality From Two Blood Extraction Kits

Abstract

A high-quality isolated sample of deoxyribonucleic acid (DNA) is essential for the success and reliability of various molecular genetic analyses. This paper presents the results of a comparative analysis of the concentration and quality of extracted DNA from 200 samples of human blood using two different commercial kits: Qiagen DNeasy Blood & Tissue Kit and High Pure PCR Template Preparation Kit – Roche Diagnostics. Concentration and quality of isolated DNA were measured by spectrophotometric (NanoDrop One) and fluorometric methods (Qubit 2.0). The average concentration of extracted DNA by the Qiagen kit was 45.06ng/µl±17.75, while with the Roche kit it was 20.97ng/µl±8.8. The quality of DNA was presented as the ratio of absorbance at 260nm and 280nm. The obtained mean value of A260/280 for the Qiagen kit was 1.8, and for the Roche kit, A260/280 was 1,66. The concentration obtained by the Qiagen kit was 2.15 times higher than the concentration obtained by the Roche kit. Concentration measured by NanoDrop was 2,33 times higher than values obtained by Qubit for the same samples. Both kits showed good quality and concentration of isolated DNA, but the Qiagen kit has superior performance in both analyzed categories. Measurement with Qubit was more specific than measurement with NanoDrop.