Efficacy of two different antibiotic solutions in preservation of fresh amniotic membrane

Abstract

Introduction. The amniotic membrane is used in transplant surgery, ophthalmology
and dermatology. Various methods have been developed to
preserve amniotic membrane: hypothermic storage, cryopreservation, lyophilization.
Transplantation of fresh amniotic membrane showed low inflammatory
response. The efficient antibiotic solutions are carefully chosen
for the hypothermic storage of amniotic membranes. The aim of this study
was to compare the efficacy of two antibiotic solutions for the hypothermic
amniotic membrane preservation and the structure of the amniotic
membrane after the preservation process.
Methods. Fifteen amniotic membranes were prepared and hypothermically
stored in penicillin solution in BSS (2000 IU/ml) while the remaining
fifteen in an antibiotic solution containing: benzylpenicillin (50 μg/ml), gentamicin
(100 μg/ml), ciprofloxacin (200 μg/ml) and fluconazole (100 μg/ml).
All amniotic membranes were microbiologically tested after preparation
and after hypothermic storage for two weeks. Histological analysis of thirty
amniotic membranes was performed after the process of preservation.
Results. Fifteen amniotic membranes were sterile after hypothermic preservation
in the penicillin solution. Also, fifteen amniotic membranes were
sterile after hypothermic preservation in the solution of antibacterial agents
(penicillin, gentamicin, ciprofloxacin) and antifungal agent fluconazole. The
amniotic membrane had a normal structure and thickness of 35.33±11.03 μm.
Conclusion. Both antibiotic solutions, one that contains only penicillin
and one that contains multiple antibacterial agents and fluconazole, provide
sterility of fresh hypothermically stored amniotic membranes for two
weeks. In the preparation of fresh hypothermically stored amniotic membrane,
a solution with multiple antibiotics is preferred. The normal tissue
structure of the amniotic membrane was histologically confirmed after the
process of preservation.